THE PRODUCTION OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR

摘要

The present invention relates to the production of human granulocyte colony-stimulating factor (hG-CSF). A recombinant plasmid (pYHM-G-CSF) containing cDNA for hG-CSF was produced and introduced into Escherichia coli (E. coli) which was developed to recombinant bacteria. The recombinant bacteria have high expression ability to 1.7g hG-CSF from 1L culture media. The refolded and purified hG-CSF has comparable biological activity of G-CSF.