| 摘要 |
<p>The present invention provides a method for increasing the sensitivity of a diagnostic test for the detection of a nucleic acid of an infectious agent in pooled samples of liquid biological material, characterized in that the nucleic acid is selectively precipitated by adding a precipitating agent, whereby the preferred precipitating agent is polyethylene glycol in a molecular weight range of 1'000 to 10'000 at a final concentration of 1 to 5 % (w/v). The precipitation efficiency may be increased by the addition of mono- or divalent salts. Preferred examples of such salts are NaCl or KCl, preferably these salts are added so that the final salt concentration is 200 to 450 mM, most preferably 270 to 325 mM salt. The invention also relates also to the use of the said method in a diagnostic test as well as to kits which are useful for practicing the said method.</p> |
