Process for aligning, adhering and stretching nucleic acid strands on a support surface by passage through a meniscus

摘要

Macromolecules such as nucleic acid strands are aligned, adhered and stretched on a support surface by passing the strands through a meniscus of a solvent containing the strands. The meniscus may be that of a solvent between two surfaces at an interface of the solvent with air. One end of a nucleic acid strand is attached to one surface which may be a glass surface and another end is free. The meniscus is moved relative to the surface to which the end is attached such as by evaporating the solvent or by moving the surface. As the nucleic acid strand passes through the meniscus, the strand elongates and aligns perpendicular to the meniscus on the surface. This method may be used in assaying, measuring intramolecular distance of and/or separating macromolecules.