METHOD FOR DETERMINING ANTIGEN AFFINITY OF HEMABSORBING ISOLATES OF AFRICAN PIG PEST VIRUSES

摘要

FIELD: medicine. SUBSTANCE: method involves setting immune reaction of radioactive labeled isolate-specific antigen (GP) having molecular mass of 110-140 kD with antibodies (protein A-sepharose CL-4B) taken from virus-specific pig sera adsorbed on solid phase. Specific radioactivity of reaction products eluated from sorbent is measured by means of special counter. Antigen affinity is calculated from a formula. 3H--glucose amine hydrochloride is used as metabolic GP marker. It is introduced into pig bone marrow cell culture infected with virus at a dose of 5-20 mcCu/ml. The GP is produced by solving infected cells in 1% triton X-100 in 0.02 M tris-HCl buffer at pH of 7.2-7.4. 22000 g ultracentrifuging and ion exchange chromatography on DEAE-cellulose is applied during 1 h with absorbed proteins of 0.125 M NaCl eluated on solving buffer. EFFECT: enhanced accuracy of method. 2 cl, 1 tblf