DISCOVERY OF AND METHOD FOR CLONING AND PRODUCING THE PspGI RESTRICTION ENDONUCLEASE

摘要

The present invention relates to the type II restriction endonuclease PspGI, obtainable from Pyrococcus species G-I-H (NEB #906), an enzyme which recognizes the DNA sequence 5' CC(A/T)GG 3' and cleaves before the first C of the recognition sequence to produce a 5 base 5' extension: 5'- &darr& CC(A/T)GG-3', 3'-GG(T/A)CC{-5', (wherein G represents guanine, C represents cytosine, A represents adenine and T represents thymine and (A/T) represents either A or T in that one position).