Seed specific biotinylated protein, SBP65, from leguminous plants

摘要

A biotinylated protein is disclosed which is obtained from the seeds of leguminous plants and which is expressed exclusively in the seeds and in no other tissue. The protein comprises at least one subunit of 50-85 kDa. Levels of the protein decrease rapidly as germination of the seed progresses. The protein does not exhibit the activity of either acetyl-CoA carboxylase or 3-methyl crotonyl-CoA carboxylase. In the pea, Pisum sativum, the protein is designated SBP65 and comprises 6-8 identical subunits, each having a molecular weight of about 65 kDa. The protein may be a useful marker for determining the germination stage of seeds.