发明名称 |
Nucleic acid analogue induced transcription of double stranded DNA |
摘要 |
RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.
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申请公布号 |
US5837459(A) |
申请公布日期 |
1998.11.17 |
申请号 |
US19960653605 |
申请日期 |
1996.05.24 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
BERG, ROLF HENRIK;BUCHARDT, DECEASED, OLE;EGHOLM, MICHAEL;NIELSEN, PETER EIGIL |
分类号 |
C12N15/09;C12P19/34;C12Q1/68;G01N33/53;G01N33/566;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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