| 摘要 |
A method for determining the presence and location of a mismatch in a target sequence using Fluorescence Resonance Energy Transfer (FRET). The method comprises contacting the target sequence with at least three labelled oligonucleotide probes capable of hybridising to the natural sequence, in juxtaposition. The central probe is labelled with a donor fluorophore and the probe either side of this is labelled with a distinct acceptor fluorophore. Hybridisation to the target sequence results in resonance between the donor fluorophore and the acceptor fluorophores. A mismatch present in the target sequence will disrupt hybridisation to that region, resulting in a significant alteration to the resonance signal. |
