发明名称 |
Polyvalent and polyclonal libraries |
摘要 |
The invention is directed to production of chimeric antibodies using display screening methods. The invention is based in part on two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods can be used to produce polyclonal libraries of chimeric antibodies for use, e.g., as diagnostic or therapeutic reagents. |
申请公布号 |
AU8755798(A) |
申请公布日期 |
1998.11.13 |
申请号 |
AU19980087557 |
申请日期 |
1998.04.03 |
申请人 |
BIOSITE DIAGNOSTICS, INC. |
发明人 |
JEFF GRAY;JOE BUECHLER;GUNARS VALKIRS |
分类号 |
G01N33/53;C07B61/00;C07K14/00;C07K16/00;C07K16/12;C12N15/09;C12N15/10;C12P21/02;C12Q1/68;C40B40/02;G01N33/543 |
主分类号 |
G01N33/53 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|