| 摘要 |
PROBLEM TO BE SOLVED: To readily and efficiently produce CD34-positive cells excellent in safety and useful for treatment of acute leukemia, tumorous disease, etc., in a short time in a high yield, by culturing human CD34-positive cells in a nutrient medium containing stroma cells derived from a mammal. SOLUTION: Human CD34-positive cells such as human CD34-strongly positive cells derived from umbilical cord blood, spinal fluid, liver, spleen and peripheral blood is cultured in a nutrient medium having abilities capable of propagating human CD34-positive cells, derived from a mammal such as mouse, containing stroma cells such as HESS-1 and HESS-5 (FERM BP-5768), and preferably containing cytokine such as interleukin-3 and hepatic cell factor to propagate the human CD34-positive cells and to provide the human CD34-positive cells in the method for producing the human CD34-positive cells. |
