METHOD OF DETERMINATION OF THERMOSTABLE ENTEROTOXIN GENE IN EXAMPLES OF ENTEROBACTERIACEAE FAMILY

摘要

FIELD: biotechnology, medicinal microbiology. SUBSTANCE: method involves dot blot hybridization using oligonucleotide probe. 30-Membered oligonucleotide that is a homolog of high-conservative site of the known ST-enterotoxins of family Enterobacteriaceae of the known nucleotide sequence: 5'-TGCTGTGAANTNTGTTGTAATCCTGCCT- -GT-3' is used as an oligonucleotide probe. The use of this oligonucleotide probe ensures to detect ST-genes without any dependence of their species. EFFECT: improved method of gene assay. 1 tbl, 5 dwg, 4 exf