| 摘要 |
<p>NTera 2/D1 (NT2) precursor cells were transfected with human β2 adrenergic receptor under the control of a neuron-specific synapsin-1 promoter. Stably transfected precursor cells did not display elevated expression of the β2 adrenergic receptor and no change was seen in the receptor coupled generation of cAMP. Upon differentiation with RA, the transfected cells displayed elevated cAMP production induced by adrenergic receptor agonists. Expression of the β2 adrenergic receptor in the neuronal NT2 cells resulted in a significantly enhanced level of neuronal differentiation compared to the untransfected NT2 cell as evidenced by the neuronal marker MAP2.</p> |
