| 摘要 |
PROBLEM TO BE SOLVED: To obtain a lymphocytic cell having cytotoxicity to tumorous cell and useful for an immunotherapeutic agent, etc., with low-toxic interleukin 15 (IL-15) by culturing the lymphocytic cell in the presence of an anti-CD3 antibody and the IL-15. SOLUTION: A physiological saline solution is added to dilute a human peripheral total blood collected by using a syringe treated with a heparin and the diluted blood is then added to a medium for density-gradient centrifugation, superposed and centrifuged to separate a lymphotic cell. The first-stage culture of the resultant lymphocytic cell is then carried out in the presence of an anti-CD3 antibody converted into the solid phase and interleukin 15 and the second-stage culture is subsequently performed in the absence of the anti-CD3 antibody converted into the solid phase and in the presence of the IL-15 to thereby provide the objective lymphocytic cell, having cytotoxicity to tumorous cells and useful as an immunotherapeutic agent, etc., suitable for the adoptive immunotherapy used for treating cancers, infectious diseases, etc., in a large amount by using the low-toxic IL-15. |
