发明名称 |
Homogeneous fluorassay methods employing fluorescent background rejection and water-soluble rare earth metal chelates |
摘要 |
Homogeneous assays for determining quantitatively the extent of a specific binding reaction can be carried out effectively on very dilute solutions using measurements of fluorescence if a fluorescence measurement scheme that is capable of rejecting short-lived background fluorescence is employed and if the fluorescent group being measured has the following properties: a. the group being measured must be a rare earth metal chelate complex combination; b. the chelate must be water-soluble; c. the complex combination must also be stable in extremely dilute aqueous solutions, that is, the measured chelate must have at least one ligand having a metal-to-ligand binding constant of at least about 1013M-1 or greater and it must have a fluorescent emission that is long-lived compared to the longest decay lifetime of ambient substances and have a half life of from 0.01 to 50 msec.
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申请公布号 |
US5830769(A) |
申请公布日期 |
1998.11.03 |
申请号 |
US19960732871 |
申请日期 |
1996.10.15 |
申请人 |
WIEDER, IRWIN;HALE, RON L. |
发明人 |
WIEDER, IRWIN;HALE, RON L. |
分类号 |
G01N33/533;(IPC1-7):G01N33/533 |
主分类号 |
G01N33/533 |
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