| 发明名称 |
Method for obtaining a modified heparinase gene |
| 摘要 |
The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.
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| 申请公布号 |
US5830726(A) |
申请公布日期 |
1998.11.03 |
| 申请号 |
US19950445342 |
申请日期 |
1995.05.19 |
| 申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
SASISEKHARAN, RAMNATH;MOREMEN, KELLEY;COONEY, CHARLES I.;ZIMMERMANN, JOSEPH J.;LANGER, ROBERT S. |
| 分类号 |
C12N15/09;C12N1/21;C12N9/88;C12N15/00;C12N15/60;C12R1/01;C12R1/20;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/09 |
| 代理机构 |
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