| 摘要 |
<p>A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (A) a first opposable component (252) comprising a chromatographic medium (256) and an application means (262) including a sample preparation means adapted to receive a sample to be assayed; and (B) a second opposable component (254) comprising a second application means (270) and an absorbing means (268) separated from the second applicator means. The first and second opposable components can be brought into opposition so as to cause liquid transfer between the components via the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analytes, as well as to permit bi-directional chromatography. The devices can be incorporated in test kits, and assay methods using the devices are also disclosed. <IMAGE></p> |
