发明名称 |
Hybridoma producing a monoclonal antibody for N-peptide |
摘要 |
The present invention relates to a sandwich immunoassay for rapidly and readily measuring N-peptide using two kinds of monoclonal antibodies recognizing different portions of the N-peptide. The method for measuring N-peptide or a precursor thereof includes the steps of: incubating a mixture containing a sample and a first monoclonal antibody recognizing a portion of N-peptide; adding a labelled second monoclonal antibody recognizing a portion of N-peptide to the mixture, followed by further incubation; and detecting the resulting antigen-antibody complex in the mixture. Alternatively, the method includes the steps of: incubating a mixture containing a sample, a first monoclonal antibody recognizing a portion of N-peptide, and a labelled second monoclonal antibody recognizing another portion of N-peptide; and detecting the resulting antigen-antibody complex.
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申请公布号 |
US5827674(A) |
申请公布日期 |
1998.10.27 |
申请号 |
US19970909002 |
申请日期 |
1997.08.08 |
申请人 |
SHIONOGI & CO., LTD. |
发明人 |
NUMATA, YOSHITO;ASADA, HIDEHISA;DOHI, KEIJI;FUKUNAGA, TAKAHIRO;TANIGUCHI, YASUSHI |
分类号 |
C07K16/26;C12N5/20;C12P21/08;G01N33/68;(IPC1-7):G01N33/53;G01N33/543 |
主分类号 |
C07K16/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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