发明名称 |
Sheets, kits and methods for detecting antigens or antibodies |
摘要 |
A detection sheet capable of detecting an antigen or antibody in a biological fluid with a high sensitivity in a simple operation, as well as a detection kit and detection method using the detection sheet. The detection sheet comprises (a) a fibrous aggregate with carried particles of a calcium phosphate compound having an average particle diameter of about 0.01 to 200 microns and a Ca/P ratio of about 1.0 to 2.0, and (b) an avidin, streptavidin or avidin derivative from which carbohydrates have been extracted immobilized on said calcium phosphate compound. After a biotinylated antigen or antibody is bonded to the immobilized avidin, streptavidin, or avidin derivative of the detection sheet, the detection sheet is contacted with a test solution to thereby bond an antigen or antibody of the test solution to the detection sheet. The detection sheet is then contacted with a solution of labeling compound capable of being specifically bonded to the antigen or antibody of said test solution to detect the thus labeled antigen-antibody complex.
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申请公布号 |
US5827669(A) |
申请公布日期 |
1998.10.27 |
申请号 |
US19960746485 |
申请日期 |
1996.11.12 |
申请人 |
NAKAYAMA, MIKIO;KITANA, TADAHIKO;ASAHI KOGAKU KOGYO KABUSHIKI KAISHA |
发明人 |
NAKAYAMA, MIKIO;KITANO, TADAHIKO;MITOH, AYUMI;OGAWA, TETSURO;HIRAIDE, TSUNEO |
分类号 |
G01N33/53;G01N33/543;G01N33/547;G01N33/551;(IPC1-7):G01N33/551 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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