发明名称 |
Vectors for cloning and modification of DNA fragments |
摘要 |
Plasmid vectors are provided which enable for the modification of double-stranded DNA which has blunt or one-base 3' overhanging termini, to include any desired four-base 5' overhang sequence, in the reading frame of choice. These vectors enable the cloning of blunt double-stranded DNA sequences or double-stranded DNA sequences having a one-base 3' overhang into a unique restriction site by blunt-end ligation or by one-base 3' overhang cohesive cloning such as T-cloning. The vectors may be amplified and subsequently excised, thereby producing modified fragments free of vector sequences.
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申请公布号 |
US5827704(A) |
申请公布日期 |
1998.10.27 |
申请号 |
US19960608437 |
申请日期 |
1996.02.28 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF MICHIGAN |
发明人 |
CEASE, KEMP B.;LOHFF, CORTLAND J. |
分类号 |
C12N15/66;(IPC1-7):C12N15/00;C12N15/11 |
主分类号 |
C12N15/66 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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