OLIGONUCLEOTIDE FOR AMPLIFYING MUTANT GENE OF HUMAN CYTOCHROME P450 IIE1

摘要

PROBLEM TO BE SOLVED: To provide a method for easily diagnosing and analyzing the presence or absence of the mutation of human cytochrome P450 IIE1 gene before the administration of a medicine, etc. SOLUTION: This oligoniucleotlde is capable of amplifying by a PCR method a nucleic acid containing the base sequence of the mutated site of the mutant gene of the human cytochrome P450 IIE1, and has a GC content of 30-70% and a base length of 8-50 bases. The presence or absence of the mutation of the human cytochrome P450 IIE1 gene is judged by detecting the nucleic acid containing the base sequence of the mutated site of the mutant gene of the human cytochrome P450 IIE1 by a PER method using the oligonucleotide as a primer in the nucleic acid containing the base sequence of the human cytochrome P450 IIE1 gene.