发明名称 |
Kits for nucleic acid amplification kit using single primer |
摘要 |
A method is disclosed for determining the presence of a polynucleotide analyte in a sample suspected of containing the analyte. The method comprises (a) forming as a result of the presence of an analyte a single stranded polynucleotide comprising a target polynucleotide binding sequence flanked by first and second polynucleotide sequences that differ from the sequence of the analyte or a sequence complementary to the analyte sequence, (b) forming multiple copies of the single stranded polynucleotide, and (c) detecting the single stranded polynucleotide. Also disclosed is a method of producing at least one copy of a single stranded polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template dependent polynucleotide polymerase an extension of a polynucleotide primer at least the 3'-end of which has at least a 10 base sequence hybridizable with a second sequence flanking the 3'-end of the single stranded polynucleotide, the second sequence being partially or fully complementary with at least a 10 base first sequence flanking the 5' end of the single stranded polynucleotide, (b) dissociating the extended polynucleotide primer and the single stranded polynucleotide, (c) repeating step a and (d) dissociating the extended polynucleotide primer and the copy of the single stranded polynucleotide.
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申请公布号 |
US5827649(A) |
申请公布日期 |
1998.10.27 |
申请号 |
US19940242931 |
申请日期 |
1994.05.16 |
申请人 |
BEHRING DIAGNOSTICS GMBH |
发明人 |
ROSE, SAMUEL;GOODMAN, THOMAS C.;WESTERN, LINDA M.;BECKER, MARTIN;ULLMAN, EDWIN F. |
分类号 |
G01N33/58;C07H21/00;C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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