Method for matrix-assisted laser desorption ionization

摘要

A method for matrix-assisted ionizing laser desorption of large analyte molecules (MALDI) in a vacuum for the generation of ions for mass spectrometric investigation of the analyte substance is provided. The matrix substance for matrix-assisted ionizing laser desorption is formed from at least two different components. One of the components is very adsorptive, as well as being decomposable thermolytically into small fractions. Additional matrix components are selected for protonation of the analyte molecules. In particular, a thin layer of nitrocellulose (also called cellulose nitrate) with a protonating substance embedded within it is particularly suitable. This layer, which is insoluble in water, adsorbs large analyte molecules from an aqueous solution at its surface.