| 摘要 |
A microplate for holding mixtures (28) of reagents and samples to be assayed by emission of light signals from the mixtures to an optical detector (30) positioned above the microplate. The microplate includes a frame (34) having a base (36) and a top portion (40) extending from the base. The base has a bottom edge (38) and the top portion (40) extends to a height (H2) above the bottom edge (38). The height (H2) is preferably 14.35 mm so that the microplate is compatible with standard tray handling equipment. The microplate also includes a plurality of wells (50) disposed in the frame (34) for holding the mixtures (28). Each well has sidewalls (44) and a well bottom (48). Each well bottom is elevated a height (E3) above the bottom edge (38) of the base (36). The height (E3) is in a range of one half to nine tenths of the height (H2). The elevation of each well bottom reduces the length of the signal path (S3) from each mixture (28) to the optical detector (30) and allows a minimum quantity of reagents to be used in each well.
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