Reagent for measurement of leukocytes and hemoglobin concentration in blood

摘要

A reagent for measurement of leukocytes and hemoglobin concentration in the blood, the reagent containing: (1) a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, (2) at least one hemoglobin stabilizer selected from the group consisting of the following (a), (b) and (c): (a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, (b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and (c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, and (3) a buffer for maintaining pH at 4 to 6. This reagent does not use a cyanogen compound, can measure the leukocyte count, and can also measure the hemoglobin concentration stably even when the liquid temperature of a blood sample varies.