Process for the analytical determination of the concentration of a part of a medical specimen

摘要

Process for the analytical determination of the concentration of a component of a medical specimen, in which a reaction of the specimen with reagents leads to a time-dependent change S(t) in a measurand (measured value) S, and the concentration C is correlated in accordance with an evaluation curve C-(X) with an input variable X derived from S(t). The evaluation curve is multi-valent at least for some of the possible X-values. In order to assign an input variable X to one of the subregions and thereby to achieve a unique correlation with a specific concentration C, the following steps are carried out: - During a training run, a discrimination algorithm is carried out at least once, in which measurements of S(t) are used to generate a discriminator set and from this a score is respectively calculated using a method of multivariate statistics, and it is checked whether the scores can be divided into separate subsets in which the concentrations are correctly assigned to the subregions of the calibration curve. The discrimination algorithm is repeated using different discriminator sets, generated from the measurements of S(t) in accordance with different generating methods, in order to establish an operative discrimination algorithm. - Measurements of S(t) are used in an analysis run to calculate in accordance with the operative discrimination algorithm an analysis score which is compared with the pivoting score, and the measurement result is assigned to one of the component curves of the calibration curve by comparison of the analysis score with the pivoting score.