A constitutive promoter from tobacco

摘要

T-DNA tagging with a promoterless beta -glucuronidase (GUS) gene generated a transgenic Nicotiana tabacum plant that expressed GUS activity constitutively. The gene fusion has been cloned and sequenced. It has been re-inserted into N. tabacum by Agrobacterium-mediated transformation. The N. tabacum DNA upstream from the GUS gene was approximately 2 kb in length and showed no homology to known sequences. This DNA, which contains a constitutive promoter, is useful in controlling the expression of exogenous genes in transgenic plants of diverse plant species.