Method for cloning and producing the PshAI restriction endonuclease

摘要

The present invention is directed to a method for cloning and producing the PshAI restriction endonuclease by (1) introducing the restriction endonuclease gene from Plesiomonas shigelloides into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the PshAI restriction endonuclease activity, and (3) purifying the PshAI restriction endonuclease from the fermented host which contains the vector encoding and expressing the PshAI restriction endonuclease activity.