Replication genes and gene products from small cryptic plasmids and methods for constructing high-copy number plasmid vectors

摘要

The replication genes of small cryptic plasmids are isolated and used to construct controlled-replication plasmid vectors with the wide range of copy numbers controlled by defined helper plasmids. Controlled-replication vectors (RAMP vectors) can reach very high level of plasmid replication, which is not lethal to host unlike runaway replication vectors.