| 摘要 |
FIELD: microbiology, biotechnology. SUBSTANCE: invention relates to the strain Pseudomonas Batumici nov. sp. 3187 as a producer of antibiotic showing antistaphylococcus activity. Strain is stored in Collection of Microbiology and Virology Institute of Academy of Sciences of the Ukraine at number 3187. Cultural-morphological characters: cells of the strain are gram-positive rods (size is 2.2 x 1.0 mcm), mobile, have two polar flagelli (length is an average 6-8 mcm), coil number is 4-6. Strain does not form inclusions of poly-β-hydroxybutyric acid. Cells grow well on usual nutrient media. On beef-extract agar: colonies are round, smooth, bright, layer-like. On beef-extract broth: uniform turbidity, yellow-green fluorescent pigment is not formed. On glucose medium: cells form yellow-brown pigment diffusing in medium that can be extracted with chloroform. Colonies are stained with yellow-brown color, being more dark that medium. Physiological-biochemical characters: strain is restrict aerobe, uses glucose by oxidative way only. Strain has activities of oxidase, arginine dehydrolase, levansaccharase and decitinase but does not reduce nitrates and does not hydrolyze gelatin and esculin. Strain does not oxidase gluconate and does not convert quinic acid to protocatechuic acid. Optimal growth temperature is 27 C, very weak growth - at 37 C and no growth at 42 C. Strain assimilates ammonium and nitrate forms of nitrogen and shows sensitivity to nitrofurantoin. Strain uses D-galactose, L-arabinose, D-mannose, fructose, trehalose, gluconate, acetic, malonic, succinic, maleic, fumaric, glutaric, lactic, isocitric, citric, aconitic, oxaloacetic, itaconic, malic, aspartic, glutamic, γ-aminobutyric and quinic acids, mannitol, inositol, glycerol, α-alanine, β-alanine, isoleucine, valine, arginine, ornithine, citrulline, betaine, sarcosine as a single carbon source. Strain does not use aliphatic alcohols, fatty acids and aromatic compounds. Antagonistic properties: strain shows activity with respect to Staphylococcus aureus, Erwinia aroideae, phytopathogenic species Pseudomonas, Xanthomonas campestris and Xanthomonas malvacearum and inhibits partially the growth of E. coli, Bac. subtilis, Corynebacterium michiganeusi, Agrobacterium tumifaciens but does not show antagonistic activity with respect to yeasts and fungi. EFFECT: bacterial strain indicated above. |
