摘要 |
Efficient methods of removing color from wood pulp are disclosed. In a preferred embodiment, the method includes the steps of preparing a wood pulp; treating the wood pulp with a cellulase, preferably a cellulase with optimum pH 3-0 - 7.0, and/or solvent, preferably methylamine, to modulate the pulp-fiber-pore-structure; and treating the wood pulp with xylanase wherein the xylanase is capable of releasing chromophores from the pulp, and extracting the wood pulp to remove chromophores. The xylanase preferably is isolated from Bacillus stearothermophilus (ATCC 55696) with molecular weight of approximately 39 kD as determined by SDS-gel electrophoresis, pH optima of pH 6.5 to 10.5, and temperature optima of between 40 DEG C and 75 DEG C; or alternately, with optimal growth at pH 5.0 to 11.0 and 40 DEG C to 75 DEG C.
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