| 摘要 |
PROBLEM TO BE SOLVED: To obtain an indole glycoside having bloodstream accelerating effect and skin whitening effect by isolation from Calanthe discolor Lindl. SOLUTION: This new indole glycoside is 3β-Dglucopyranosyl(1→6)β- Dglucopyranosylindole (hereafter referred to as calanthoside A). This glycoside is obtained by the following process: rootstocks of Calanthe discolor Lindl. are sliced, and subjected to hot extraction with methanol followed by partition extraction with ethyl acetate and water, and the resultant aqueous phase is put to reversed-phase silica gel column chromatography followed by successive elution with water and methanol to afford a methanol elute, which, in turn, is subjected to two-stage fractionation by normal-phase silica gel column chromatography followed by repeated separation using reversed-phase high- performance liquid chromatography. Calanthoside A significantly raises rat skin's blood circulation promoting effect, having melanogenetic enzyme tyrosinase activity-suppressive effect, thus being usable in hair growers and skin whitening agents. |
