| 摘要 |
PROBLEM TO BE SOLVED: To obtain an indole glycoside having blood circulation promoting effect by isolation from Calanthe discolor Lindl. SOLUTION: This new indole glycoside is 2-S-β-Dglucopyranosyl3-o-β- Dglucopyranosyl-2-mercaptoindole (hiereafter referred to as calanthoside B). This glycoside is obtained by the following process: rootstocks of Calanthe discolor Lindl. are sliced, and subjected to hot extraction with methanol followed by partition extraction with ethyl acetate and water, and the resultant aqueous phase is put to reversed-phase silica gel column chromatography followed by successive elution with water and methanol to afford a methanol elute, which, in turn, is subjected to reversed phase silica gel column chromatography followed by normal-phase silica gel column chromatography and then separation by reversed-phase silica gel chromatography followed by reversed-phase highperformance liquid chromatography. Calanthoside B raises rat skin's blood circulation promoting effect, thus being hopeful of applications such as hair growers. |
