摘要 |
The invention concerns a method and a reagent for determining lipase in the presence of a colored substrate and at least one N-substituting carboxamide derivative or a mixture of the general formula (I) or (Ia), in which R1 and R2 independently represent hydrogen or a saturated or non-saturated, substituting or non-substituting hydrocarbon residue with 2 to 24 carbon atoms, Z a saturated or non-saturated, substituting, cyclic or linear-chain hydrocarbon residue with 1 to 10 carbon atoms, X an atom or atom group with positive charge and n a figure ranging from 1 to 3. Specially tetranatrium-N-(1,2-dicarboxylethyl)-N-alkyl-sulfo-succinami de or a mixture containing such compound has proven to be quite advantageous in eliminating unspecified reactions during the determination of lipase in a biological test sample.
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