| 摘要 |
Methods for making chromosome-specific libraries from single chromosomes are disclosed in which a single flow-sorted chromosome (or subchromosomal fragment) is efficiently collected, and DNA extracted from the chromosome is amplified, e.g., by PCR. After they are produced, the resulting libraries are screened, e.g., by in situ hybridization or hybridization to a chromosome spot blot, to identify libraries that arise from the chromosome (or subchromosomal fragment) of interest. The single-chromosome amplification libraries and individual DNA sequences from the libraries are useful, for example, for cytogenetic analysis and cancer diagnostics.
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