| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new enzyme, comprising a sphingolipid ceramide N-deacylase capable of acting on an intramolecular ceramide in a sphingolipid and producing a lysosphingolipid and a fatty acid and useful for the production, etc., of (lyso)sphingolipids. SOLUTION: This new sphingolipid ceramide N-deacylase has actions on the reaction with an intermolecular ceramide in a sphingolipid, hydrolysis of the intramolecular ceramide into a sphingosine base and a fatty acid and production of a lysosphingolipid and a fatty acid, is capable of specifically acting on a ceramide, a sphingoglycolipid and a sphingolipid, has 8-10 optimum pH and is used for the production, etc., of a lysosphingolipid and sphingolipids, useful for introducing a saccharide chain into a protein and used as a substrate for measuring activities of an enzyme related to the sphingolipids, a ligand for an affinity chromatography, an antigen for producing an antibody and for the development, etc., of a therapeutic agent for atopic dermatitis. The enzyme is obtained by culturing a microorganism, collected from skin of a patient suffering from the atopic dermatitis and capable of producing the sphingolipid ceramide N-deacylase and separating the enzyme from the resultant cultured product. |
