摘要 |
Methods of forming cationic liposome/nucleic acid complexes in which the complexes have a mean diameter of about 200 to about 300 nm are provided. The complexes are formed by combining a first solution of preformed cationic unilamellar liposomes with a mean diameter of from 100 to 150 nm, with a second solution of nucleic acid. Each of the solutions are equilibrated prior to mixing to temperatures of from 0 ~C to about 12 ~C, preferably about 2 ~C to about 7 ~C. The preformed cationic liposomes are typically prepared from an unsaturated cationic lipid, for example DODAC, DOTAP, DOTMA, DODAP, DMRIE, DORI, DOSPA and combinations thereof, and a neutral lipid, for example DOPE or cholesterol. The combination of the first and second solutions is typically carried out by gentle mixing over ice for a period of time of from about 10 to about 60 minutes.
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申请人 |
INEX PHARMACEUTICALS CORP. |
发明人 |
HOPE, MICHAEL J.;WASAN, ELLEN K.;REIMER, DOROTHY L.;BALLY, MARCEL B.;AHKONG, QUET FAH |