REPLICATION GENES AND GENE PRODUCTS FROM SMALL CRYPTIC PLASMIDS AND METHODS FOR CONSTRUCTING HIGH-COPY NUMBER PLASMID VECTORS

摘要

The replication genes of small cryptic plasmids are isolated and used to construct controlled-replication plasmid vectors with the wide range of copy numbers controlled by defined helper plasmids. Controlled-replication vectors (RAMP vectors) can reach very high level of plasmid replication, which is not lethal to host unlike runaway replication vectors.