THERMOLABILE URACIL-DNA-GLYCOSYLASE, PROCESS FOR ITS PREPARATION AND USE FOR REMOVING URACIL FROM DNA

摘要

Thermolabile enzyme with uracil-DNA-glycosylase activity which is in particular characterized by a high degree of purity, short half-lives and a content of contaminating foreign activities of less than 2%, a process for its isolation as well as the use thereof to remove the base uracil from DNA and in particular from PCR products containing uracil. The enzyme is obtainable from gram-positive microorganisms such as e.g. Arthrobacter or Micrococcus.