| 摘要 |
A self-staining microscopic slide designed for immediate staining and viewing of cells in biological fluid and tissue samples, preferably with an epi-fluorescence microscope. The pre-prepared microscope slide preferably has a supravital fluorescent stain applied thereon, which is overlaid with a transparent tape or film. During use, the film is peeled back to expose the stain so that a sample can be applied thereon for intermixture therewith. The film is then replaced over the stained sample to act as a cover slip for immediate viewing. Living cells and microorganisms are rendered dramatically visible and cellular dysmorphology can be readily ascertained. The time and cost associated with preparing a fixed and sectioned sample is completely avoided as well as the problem of artifacts and sample fragmentation found in fixed preparations. The slide can include reference standards to facilitate microscope focusing, and to allow measurements of cells and microorganisms. An alternate embodiment provides a flexible microscope slide which can be folded over such that a viewing portion of the slide can be placed against a specimen to obtain a sample directly therefrom without the need of transferring devices, thereby reducing biological hazards. This novel slide permits on-site, point-of-care screening in a matter of minutes of any biological fluid or tissue sample for presence of infectious agents.
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