发明名称 |
DNA encoding human endothelin-3, and use thereof |
摘要 |
A cloned DNA molecule is disclosed. The DNA molecule encodes a human endothelin-3 protein having the amino acid sequence: Glu-Gly-Ala-Pro-Glu-His-His-Arg-Ser-Arg-Arg-Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp-Ile-Asn-Thr-Pro-Glu or any portion of said amino acid sequence containing Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp. Also disclosed is a method of producing mature endothelin-3 protein. In the method, the host cell is cultured, allowing mature endothelin-3 to accumulate in the culture medium, and the mature endothelin-3 protein is separated from the culture medium.
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申请公布号 |
US5811263(A) |
申请公布日期 |
1998.09.22 |
申请号 |
US19960645771 |
申请日期 |
1996.05.14 |
申请人 |
TAKEDA CHEMICAL INDUSTRIES, LTD. |
发明人 |
MASAKI, TOMOH;YANAGISAWA, MASASHI;INOUE, AKIHIRO |
分类号 |
A61K38/00;C07K14/575;C12N1/21;C12N15/12;C12N15/16;(IPC1-7):C12N1/21;C12N5/10;C12N15/63 |
主分类号 |
A61K38/00 |
代理机构 |
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主权项 |
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地址 |
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