Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator
摘要
Biologically active human tissue-type plasminogen activator is produced in a host cell culture by removing from the medium of the culture all medium components which are detrimental to recovery and activity of the plasminogen activator. For example, cross filtration may be used to carry out medium exchange during culture, or the detrimental components may be removed prior to culturing. The cells retain their expression capability after exclusion of the harmful substances so that production of active plasminogen activator can be effected.
申请公布号
DE3718939(C2)
申请公布日期
1998.09.17
申请号
DE19873718939
申请日期
1987.06.05
申请人
GENENTECH, INC., SOUTH SAN FRANCISCO, CALIF., US
发明人
ARATHOON, WILLIAM R., SAN FRANCISCO, CALIF., US;BUILDER, STUART E., BELMONT, CALIF., US;LUBINIECKI, ANTHONY S., RESTON, VA., US;REIS, ROBERT D. VAN, REDWOOD CITY, CALIF., US