AN EX VIVO ANIMAL OR CHALLENGE MODEL AS METHOD TO MEASURE PROTECTIVE IMMUNITY DIRECTED AGAINST PARASITES AND VACCINES SHOWN TO BE PROTECTIVE IN SAID METHOD

摘要

The invention relates to an ex vivo animal or challenge model as a method to identify protective (recombinant) proteins and rapidly measure protective immunity in intestinal segments, directed against parasites and vaccines directed against parasitic infections. The invention further relates to vaccines directed against infection with parasites, such as Fasciola hepatica, which vaccines contain protective (recombinant) proteins identified and shown to be protective in studies using the ex vivo model. The invention further relates to protective (recombinant) proteins derived from newly excysted juveniles (NEJ) of Fasciola hepatica, and relates to the protective (recombinant) protein or fragments thereof corresponding to an NEJ protein with an apparent molecular weight of 32 kD and N-terminal amino acid sequence comprising the sequence XXDVSWPFWDRMYNY, in which the amino acids are given in the one letter code.