发明名称 |
Method of affinity purifying antibodies |
摘要 |
The present invention relates to a method of affinity purifying antibodies consisting of the following steps: A) homogenizing Helminth parasite worms forming a mixture; B) incubating the mixture of adult Helminth parasite worm homogenate with pre-swelled CNBr-activated Sepharose 4B in coupling buffer; C) first washing the mixture with blocking buffer; D) second washing the mixture with acetate buffer and coupling buffer; E) third washing the mixture with Phosphate Buffered Solution; F) pouring the mixture into a chromatography column; G) allowing the mixture to settle; H) first passing, by gravity, a solution of saturated ammonium sulfate precipitated antibodies in loading buffer through the chromatography column; I) second passing, by gravity, loading buffer through the chromatography column until thoroughly washed; and J) second passing, by gravity, chromatography column elution buffer through the chromatography column which functions to remove and the purified antibodies.
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申请公布号 |
US5808009(A) |
申请公布日期 |
1998.09.15 |
申请号 |
US19960710845 |
申请日期 |
1996.09.23 |
申请人 |
NORTH DAKOTA STATE UNIVERSITY RESEARCH FOUNDATION |
发明人 |
ROBINSON, MICHAEL;GUSTAD, THOMAS RUSSELL;MEINHARDT, STEVEN WAYNE |
分类号 |
C07K14/435;C07K16/18;(IPC1-7):A23J1/00;C07K16/00 |
主分类号 |
C07K14/435 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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