| 摘要 |
PROBLEM TO BE SOLVED: To enable fermentation in the presence of an organic solvent in high concentration in a high state of bacterium activity, namely in a large number of viable cells, to prevent contamination with various germs and to improve fermentation productivity. SOLUTION: A bacterium is cultured under restriction of oxygen in a two phase-based culture solution comprising an aqueous solution (water phase) containing an electron acceptor compound (preferably 10-100mM addition concentration) such as a sulfate or a carbonate in anaerobic respiration and a component such as glucose required for proliferation of bacterium and a slightly water-soluble organic solvent (organic solvent phase). Preferably the culture solution contains >=2mM of sodium nitrate and >=0.2(W/V)% of glucose. |
