发明名称 |
Diagnostic assays using soluble endothelial cell protein C/activated protein C receptor |
摘要 |
Plasma EPCR has been isolated, characterized and shown to block cellular protein C activation and APC anticoagulant activity. Plasma EPCR appears to be about 43,000 daltons and circulates at approximately 100 ng/ml (98.4+/-27.8 ng/ml, n=22). Plasma EPCR bound activated protein C with an affinity similar to that of recombinant soluble EPCR (Kdapp approximately 30 nM), and inhibits both protein C activation on an endothelial cell line and APC anticoagulant activity in a one-stage factor Xa clotting assay. Soluble plasma EPCR appears to attenuate the membrane-bound EPCR augmentation of protein C activation and the anticoagulant function of activated protein C. Soluble EPCR has also been detected in urine. Levels of soluble EPCR can rise in inflammatory disease associated with vascular injury and appear to be correlated with inflammation and disease states associated with abnormal coagulation. Since EPCR expression is restricted to larger vessels and is usually negative in cappillaries, these observations provide a mechanism for analyzing injury/stimulation of large vessel endothelial cells.
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申请公布号 |
US5804392(A) |
申请公布日期 |
1998.09.08 |
申请号 |
US19970884203 |
申请日期 |
1997.06.27 |
申请人 |
OKLAHOMA MEDICAL RESEARCH FOUNDATION |
发明人 |
ESMON, CHARLES T.;STEARNS-KUROSAWA, DEBORAH J.;KUROSAWA, SHINICHIRO |
分类号 |
C07K14/705;C07K14/74;C07K16/28;C07K16/38;C12N15/00;C12P21/08;G01N33/53;G01N33/564;G01N33/566;G01N33/68;G01N33/86;(IPC1-7):G01N33/53 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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