| 摘要 |
PROBLEM TO BE SOLVED: To provide a producing method excellent in productivity of a malonic acid monoester useful as a synthetic intermediate for various chemicals, pharmaceutical agents, agrochemicals, etc. SOLUTION: A cyanoacetic acid ester represented by the formula NCCH2 COOR (R is an alkenyl group, an aryl group, an aralkyl group or a 1-20C alkyl group) is treated with a cultured product, a cell or a cell-treated material of a microorganism belonging to the genus Corynebacterium or Gordona and having nitrilase activity to produce the objective malonic acid monoester represented by the formula HOOCCH2 COOR (R is mentioned above). |
