发明名称 |
Vectors and process for producing high purity 6,12-dideoxyerythromycin A by fermentation |
摘要 |
A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A.
|
申请公布号 |
US5801032(A) |
申请公布日期 |
1998.09.01 |
申请号 |
US19960691162 |
申请日期 |
1996.08.01 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
STASSI, DIANE L.;MAINE, GREGORY T.;POST, DAVID A.;SATTER, MARK T. |
分类号 |
C12N15/09;C12N5/10;C12N9/06;C12N9/10;C12N15/76;C12N15/90;C12P19/62;(IPC1-7):C12N15/00;C12N1/14;C12N15/63 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|