发明名称 |
Methods and vectors for site-specific recombination |
摘要 |
The present invention provides methods for site-specific recombination in a cell, as well as vectors which can be employed in such methods. The methods and vectors of the present invention can be used to obtain persistent gene expression in a cell and to modulate gene expression. One preferred method according to the invention comprises contacting a cell with a vector comprising an origin of replication functional in mammalian cells located between first and second recombining sites located in parallel. Another preferred method comprises, in part, contacting a cell with a vector comprising first and second recombining sites in antiparallel orientations such that the vector is internalized by the cell. In both methods, the cell is further provided with a site-specific recombinase that effects recombination between the first and second recombining sites of the vector.
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申请公布号 |
US5801030(A) |
申请公布日期 |
1998.09.01 |
申请号 |
US19950522684 |
申请日期 |
1995.09.01 |
申请人 |
GENVEC, INC. |
发明人 |
MCVEY, DUNCAN L.;KOVESDI, IMRE |
分类号 |
C12N15/09;C12N9/24;C12N15/34;C12N15/90;(IPC1-7):C12N15/63;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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