DNA sequencing apparatus and method for a small format gel with a magnified readout

摘要

The time, difficulty, and expense of running DNA sequencing gels is substantially reduced by running the DNA sequence in a minigel of approximately 8x11 cm at a reduced electrophoretic voltage and without preheating the buffer solution in contact with the gel. The image produced from the sequence gel or autoradiograph is then scanned with a CCD line camera. The pixel map of the autoradiograph image is then magnified using software techniques within the computer so that it is displayed on the CRT screen at an increased scale permitting easy visual separation between the DNA bands.