METHOD FOR INCREASING THERMOSTABILITY IN CELLULASE EMZYMES BY REMOVAL OF THE CELLULOSE BINDING DOMAINS

摘要

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostabilit y and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide o f the full size E1. The second method of producing the new modified E1 is genetic truncatio n of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.